INTERNATIONAL RESEARCH JOURNAL OF SCIENCE ENGINEERING AND TECHNOLOGY

( Online- ISSN 2454 -3195 ) New DOI : 10.32804/RJSET

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SELECTION OF PEG -6000 INDUCED DROUGHT TOLERANT CALLUS LINES OF BREAD WHEAT (TRITICUM AESTIVUM L.)

    3 Author(s):  V.S. JAT, S. S. PUNIA, J. R. DHAYAL

Vol -  8, Issue- 2 ,         Page(s) : 90 - 95  (2018 ) DOI : https://doi.org/10.32804/RJSET

Abstract

Mature embryo of bread wheat (Triticum aestivum L.) genotype Hindi 62 was aseptically cultured on MS medium supplemented with combinations of 2,4-D and kinetin to induce callusing. The treatment 2,4-D + kinetin (10+0) proved to be better in terms of earliest days to callus induction and callus growth and this protocol combination along with 0% PEG, 10% PEg and 20% PEG was applied on ten genotypes of bread wheat (Triticum aestivum L.) viz., Hindi 62, Raj 3077, Kailash, PBN 151, C 306, Raj 3765, Kalyan Sona, K 9708, GW 190 and HD 2329 for selection of PEG- 6000 induces drought tolerant callus lines of wheat.

Barakat MN & Abdel Latif TH 1995 In vitro selections of drought tolerant lines in wheat effect of PEG: Alexandria J. Agri. Res. 40 (1): 77.
Bi RM Kou M Chen LG Mao SR & Wang HG 2007 Plant regeneration through callus initiation from mature embryo of Triticum. Plant Breeding 126, 9-12
Chandrasekhara Reddy P Vajranabhaiah SN & Prakash AH 1994 Varietal response of upland rice calli to polyethylene glycol (PEG-6000) stress. Adv. Plant Sci. 7: 12.

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