The hydroalcholic extract of the roots of Phytolacca acinosa were investigated for their in vitro phytochemical, antibacterial and antifungal activities.The preliminary phytochemical screening of plant extract shows the existence of tannins, flavonoids, carbohydrates, protein, sulphur, proteins, polyphenol, tanican dphenolic compounds. The extract of Phytolacca acinosa showed significant antibacterial and antifungal activities when tested against eight bacterial strains and four fungal strains by disc diffusion method. The results indicated that the extract inhibited the growth of selected bacteria and fungi. Thin layer chromatography performed on the root extracts confirmed the presence of various phytochemicals. The findings obtained in the present study suggest that root extract of Phytolacca acinosa could be use flint eating diseases caused by the tested organisms.