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SELECTION OF PEG -6000 INDUCED DROUGHT TOLERANT CALLUS LINES OF BREAD WHEAT (TRITICUM AESTIVUM L.)
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Author(s):
V.S. JAT, S. S. PUNIA, J. R. DHAYAL
Vol - 8, Issue- 2 ,
Page(s) : 90 - 95
(2018 )
DOI : https://doi.org/10.32804/RJSET
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Abstract
Mature embryo of bread wheat (Triticum aestivum L.) genotype Hindi 62 was aseptically cultured on MS medium supplemented with combinations of 2,4-D and kinetin to induce callusing. The treatment 2,4-D + kinetin (10+0) proved to be better in terms of earliest days to callus induction and callus growth and this protocol combination along with 0% PEG, 10% PEg and 20% PEG was applied on ten genotypes of bread wheat (Triticum aestivum L.) viz., Hindi 62, Raj 3077, Kailash, PBN 151, C 306, Raj 3765, Kalyan Sona, K 9708, GW 190 and HD 2329 for selection of PEG- 6000 induces drought tolerant callus lines of wheat.
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